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Figure 5 Antibodies to MDA–apoB-100 Peptides Block oxLDL-Induced Monocyte MCP-1 Production In Vitro
(A) MCP-1 levels in conditioned medium from monocytes incubated with titrated concentrations of IEI-E3 (shaded squares), 2D03 (solid squares), or FITC-8 control IgG1 (open squares) in the presence of oxLDL-containing human serum. The graph shows mean ± SD of triplicate values from a representative experiment. (B)
MCP-1 messenger ribonucleic acid (mRNA) relative to 18S mRNA levels in human monocytes incubated with 60 µg/ml 2D03, IEI-E3, or FITC-8 in the presence of oxLDL-containing human serum. Graphs show mean ± SD of triplicate values using cells obtained from 3 different donors (n = 9). ***p < 0.001 versus FITC-8. IgG = immunoglobulin G; MCP = monocyte chemoattractant protein; oxLDL = oxidized low-density lipoprotein; other abbreviations as in Figure 2.
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