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Figure 4 PDI Over-Expression Has a Protective Effect in the In Vivo Acute MI Mouse Model
Adenoviral vectors expressing both PDI and green fluorescent protein (GFP) (AdPDI/GFP) or GFP only (Adnull/GFP) were administered into the ventricular wall into C57/BL6 mice. A set of animals were killed 48 h after adenoviral administration to determine the amount of transgene expression. The remaining animals were subjected to left coronary artery ligation 48 h after viral administration. One week after the surgical procedure, mice were killed for analysis. (A) Adenovirus mediated over-expression of PDI-wt in C57/BL6 mice. Mice were killed 48 h after adenoviral injections. Western-blot analyses of GFP and PDI expression were performed with total lysates of homogenized myocardial tissue. (B) Immunohistochemical analysis of GFP and PDI expression in the injected hearts (original magnification x20). (C) Analysis of infarct extension, both in terms of extension and thickness, in AdPDI/GFP-injected hearts with respect to the controls. (D) Analysis of the apoptotic rate in injected hearts with respect to the controls. (E) Echocardiographic analysis of the left ventricular end-diastolic diameter (LVEDD) parameter in AdPDI/GFP-injected hearts with respect to the controls. (F) Echocardiographic analysis of the left ventricular end-systolic diameter (LVESD) parameter in AdPDI/GFP-injected hearts with respect to the controls. Abbreviations as in Figures 1 and 2.
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