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Figure 1 Analysis of PDI Expression on Autoptic Human Infarcted Hearts
(A) Semiquantitative reverse transcriptase polymerase chain reaction showing higher expression of Protein Disulfide Isomerase (PDI) transcript in the ribonucleic acid (RNA) extracted close to the infarcted area, with respect to the RNA extracted in the area remote from the infarct. The PDI expression in RNA extracted from a nonischemic heart as well as a reaction made with no RNA were used as controls. (B) Immunohistochemical expression of PDI protein in the infarcted area (original magnification x40). (C) Immunohistochemical expression of PDI in the area remote from the infarct (original magnification x40). (D) Double staining for PDI protein and terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) in the infarcted area: only a few cardiomyocytes expressing high levels of PDI were TUNEL positive, as indicated by an asterisk (original magnification x40). (E) Correlation between PDI expression and apoptotic rate in the peri-infarct region. (F) Correlation between PDI expression and congestive heart failure (CHF) signs. (G) Correlation between PDI expression and biventricular enlargement.
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