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Figure 1 Effects of Intact IgG/Antibody Fragments on Isolated, Field-Stimulated Rat Cardiomyocytes
Changes of calcium transients and systolic cell shortening during superfusion (% changes from baseline; mean ± SEM; n = 6 experiments for each patient's/control sample) with: intact immunoglobulin (Ig)G from plasma of healthy blood donors (control IgG) and intact IgG from dilated cardiomyopathy (DCM) patients (DCM-IgG); F(ab')2 fragments of control IgG and of respective DCM-IgG; intact DCM-IgG after pre-incubation with the F(ab')2 fragments of control subjects and of respective DCM patients; intact DCM-IgG after pre-incubation of cardiomyocytes with human Fc fragments; and goat-anti-human-F(ab')-IgG after pre-incubation of cardiomyocytes with F(ab')2 fragments of control IgG and of respective DCM patients. +++p < 0.001 significantly different from control IgG; ***p < 0.001 significantly different from DCM-IgG.
