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Figure 3 Effects of Fsk on WT and R14C I
Ks Channels
(A) Forskolin (Fsk), 1 µM, resulted in an increase in current amplitude (green) for both wild-type (WT) and R14C I
Ks channels. (B) The Fsk resulted in an  60% increase in current recorded at the end of a 1-s depolarization pulse to +20 mV in both WT (red) and R14C (blue)
I
Ks channels. (C) The Fsk caused a small increase in current density measured after 4-s depolarization steps (protocol as shown in Figure 2A) for both WT and R14C IKs channels. (D) The Fsk caused a leftward shift in the V0.5 of the voltage-dependence of activation for WT (red dashed line = control, red boxes = + Fsk) and R14C (blue dashed line = control, blue boxes = + Fsk) I
Ks channels. Both shifts were statistically significant (p < 0.05). The Fsk caused an acceleration of the rates of activation (E) and a deceleration of the rates of deactivation (F) for both WT and R14C I
Ks channels. Symbols in panels E and F are the same as for panel D.
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