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Figure 1 Immunohistochemistry of the human mitral degenerative valves and calcified tricuspid and bicuspid aortic valves for calcification, cartilage, and MicroCT scan. Control valve, degenerative mitral valve (arrow points to hypertrophic chondrocytes), calcified aortic valves (arrow points to positive stain), and bicuspid aortic valve (arrow points to positive stain) (magnification 25x). (A) Alizarin red stain. (B) Alcian blue stain. (C) MicroCT scan (gray area is the soft tissue, white area is calcification, blue is the background).
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