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Figure 2 Mitochondrial inner membrane potential (_m) in neonatal rat cardiac myocytes. (A) FL-2 histograms of fluorescence-activated cell sorter data from tetramethylrhodamine ethyl ester (TMRE)-loaded cells are shown. H = cells exposed to 100 µmol/l H₂O₂ for 1 h; SFA = cells pretreated with 100 µmol/l SFA for 30 min followed by 100 µmol/l H₂O₂ for 1 h. In all of the histograms, the position of the major population of control group is indicated by a vertical dashed line. Results are representative data from at least three independent experiments. (B) Representative data of the percentage of cells that maintain high (>300) TMRE fluorescence. Cells were pretreated with various concentrations of SFA for 30 min, followed by 100 µmol/l H₂O₂ for 1 h. Serofendic acid preserved _m in a concentration-dependent manner. (C) Summarized data of the percentage of cells that maintain high (>300) TMRE fluorescence. Cells were pretreated with various drugs for 30 min followed by 100 µmol/l H₂O₂ for 1 h. SFA = 100 µmol/l SFA; DZ = 100 µmol/l diazoxide; 5-HD = 500 µmol/l 5-hydroxydecanoate. *p < 0.05 versus H. #p < 0.05 versus corresponding 5-HD–absent group. Other abbreviations as in Figure 1.

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