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Figure 1 (A) The terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining (left panels) and the nuclear counterstaining by 4',6-diamidino-2-phenylindole (DAPI) (right panels) in neonatal rat cardiac myocytes. C = control cells; H = cells exposed to 100 µmol/l H2O2 for 16 h; SFA = cells pretreated with 100 µmol/l serofendic acid for 30 min followed by 100 µmol/l H2O2 for 16 h. Scale bars = 20 µm. (B) Quantitative determination of TUNEL-positive nuclei (n = approximately 3 to 4 for each group from two independent cultures). An average of 200 to 400 nuclei from random fields was analyzed in each sample. (C) Cellular viability evaluated by MTS assay (n = 13 for each group from two independent cultures). The H2O2 treatment was 3 h in this experiment. *p < 0.05 versus group H.
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