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Figure 7 Placental growth factor (PlGF) gene expression and angiogenesis in ischemic myocardium. (A) Relation between the duration of ischemia and the PlGF gene expression three days after surgery in mouse models of myocardial infarction (MI, as permanent occlusion) and ischemia-reperfusion (IR). Bars represent PlGF mRNA levels of infarct heart tissue. Placental growth factor mRNA expression levels were significantly increased in both in 60 min. Ischemia-reperfusion and MI models compared with sham operated mice. (B) Relation between the duration of ischemia and the blood vessel density in the marginal area of infarct myocardium in mouse models of MI and IR. Heart tissue was sampled seven days after surgery. Bars represent alpha-smooth muscle actin-positive blood vessel counts in infarct lesion per field under x400 microscopy. Blood vessel densities in both MI and IR are significantly increased compared with sham, and blood vessel density in MI is significantly higher (2.5x) compared with IR. *p < 0.001 for MI and IR vs. sham; **p < 0.001 for MI vs. IR; ***p < 0.01 for IR 60 min vs. sham.
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