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Figure 6 In situ detection of reactive oxygen species in rat myocardium after repeated I/R. Representative fluorescent photomicrographs of confocal microscopic sections of myocardium labeled with the oxidative dye dihydroethidium (DHE). Red fluorescence is generated when dihydroethidium DHE is oxidized to EtBr by O2 –. At identical laser settings, fluorescence in the HO-1–treated animals is markedly decreased compared with lacZ-treated controls and is similar to the sham controls.
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