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Figure 4 (A) Basal lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) messenger ribonucleic acid (mRNA) expression in pools of separated lymphocytes (1 to 3) and monocytes (4 to 6), derived from control subjects (1 and 4) and stable (2 and 5) and unstable (3 and 6) angina patients. The LOX-1 mRNA was analyzed by reverse-transcriptase polymerase chain reaction. The LOX-1 mRNA levels were normalized to the levels of beta-actin mRNA. Data illustrated on the bar graph represent the mean ± SD of six different experiments. *p < 0.001 vs. lymphocytes (1 to 3). (B) The LOX-1 protein expression in pools of separated lymphocytes (1 to 3) and monocytes (4 to 6) derived from control subjects (1 and 4) and stable (2 and 5) and unstable (3 and 6) angina patients. Lymphocytes and monocytes were separated as described in the Methods section. The LOX-1 protein expression was analyzed by flow cytometry with a specific anti-LOX-1 monoclonal antibody. Results are expressed as mean fluorescence intensity (MFI) and are means ± SD of experiments performed in triplicate on six separate occasions. *p < 0.001 vs. lymphocytes (1 to 3).
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