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Figure 4 Histological and Biochemical Analyses of Skeletal Muscles in BMT Mice
(A) Transverse sections of the deep region in the tibialis anterior muscle were stained for adenosine triphosphatase activity (top) and for succinate dehydrogenase (SDH) activity (bottom). Scale bars indicate 50 µm. Quantitative analysis results for muscle type distribution and SDH activity are shown in B and C, respectively. (D) Total SDH activity from tibialis anterior muscle measured by enzymological methods (n = 3 for WT-BMT and n = 5 for Pres-BMT mice). (E and F) Endothelial cells were identified by staining with von Willebrand factor antibody. Capillary density was calculated by counting the numbers of capillaries in 20 random high-power fields (x400 power). Scale bars indicate 50 µm. Abbreviations as in Figures 1 and 2.
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