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Figure 1 Plasma ACE2 Enzymatic Assay Specificity
(A) rACE2 (25 ng/ml) was mixed with 30% commercial human plasma in the presence of different concentrations of the specific ACE2 inhibitor DX600, and ACE2 activity was determined after incubation for 18 h. Data are expressed as the percentage of ACE2 activity of rACE2. (B) Thirty percent human plasma (patient sample) was pre-incubated with either DX600 (1 µmol/l), anti-human ACE2 polyclonal antibody, or isotype-matched control antibodies for 30 min and then ACE2 activity was determined at 4 and 18 h. One of 3 representative experiments is shown. ACE = angiotensin-converting enzyme; IgG = immunoglobulin G; rACE2 = recombinant angiotensin-converting enzyme 2 activity; SACE = soluble angiotensin-converting enzyme.
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