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Figure 2 MT Protects Cardiomyocyte Apoptotic Death From a 24-h Exposure to Ang II (100 nmol/l) and Role of NOX-Dependent Peroxynitrite Generation in Ang II Apoptotic Effect
(A) Deoxyribonucleic acid (DNA) fragmentation by enzyme-linked immunosorbent assay. (B) 3-NT formation by Western blotting. (C) Fluorescent imaging of peroxynitrite in the cultured cardiomyocytes exposed to Ang II for 6 h. Top row, phase contrast images; bottom row, fluorescent images. (D) Cardiomyocytes exposed to Ang II for 24 h with or without 1-h pre- and coincubation with urate (peroxynitrite inhibitor), MnTMPyP (superoxide inhibitor), L-NAME (nitric oxide synthase inhibitor), and apocynin (NOX inhibitor). (E) Western blotting for the membrane translocation of NOX p47phox induced by a 12-h Ang II exposure. Results are presented as relative to control with pooled results from 3 separate experiments with triple samples for each. *p < 0.05 versus control; #p < 0.05 versus Ang II alone. L-NAME = NG-nitro-L-arginine methyl ester; MnTMPyP = Mn(111) tetrakis 1-methyl 4-pyridylporphyrin pentachloride; other abbreviations as in Figure 1.
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