Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 6 IGF-1 and eNOS Mediate the Effects of Exercising on Survival Proteins
(A) Effects of 21 days voluntary running on cardiac expression of insulin-like growth factor (IGF) mRNA (n = 8). (B) Regulation of telomerase activity by treatment with growth hormone (GH) (2.5 µg/g once per day for 7 days) and mouse IGF-1 (1.5 µg/g 3 times per day for 2 days) (n = 4). (C) Representative Western blots and (D) quantification of phosphorylated Akt (pAkt) and (E) phosphorylated endothelial nitric oxide synthase (peNOS) (n = 4 per group). Effects of 21 days of running in B6.129S WT and eNOS-deficient (eNOS–/–) mice compared with sedentary controls on (F) cardiac telomerase activity, (G) expression of TRF2, (H) p16, (I) Chk2, and (J) p53 (n = 8 per group). Standardization for GAPDH. *p < 0.05, **p < 0.01, ***p < 0.001. Abbreviations as in Figures 1, 2, and 5.
|
