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Figure 1 Expression and Subcellular Localization of AAMP
(A) Two-photon laser-scanning microscopy of sparse smooth muscle cells (left) detecting intensified angio-associated migratory cell protein (AAMP) distribution (green) at the cellular membrane (arrows), whereas dense cells (right) contain less AAMP without distinctive distribution patterns. (B) Subcellular fractioning: the cellular membrane contains >50% of total cellular AAMP; as expected by fluorescence microscopy, the cellular nuclei did not contain AAMP. (C) Flow cytometric analysis of AAMP expression treated or untreated with lysis buffer confirmed the results of the subcellular fractioning experiments (red: nonimmune serum, green: without Tween, blue: with Tween). (D) Computed AAMP protein sequence analysis revealed a potential transmembrane domain between amino acids 322 and 345. FITC = fluorescein isothiocyanate.
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