Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 1 Example of Flow Analysis
Panel A shows the forward scatter (FSC), side scatter (SSC), and the gates used in the analysis. Panel B shows the staining with the isotype controls for the CD34- and KDR-specific antibodies, and panel C shows the specific staining with these antibodies. Panel D shows the CD34+/KDR+ cells stained with the osteocalcin (OCN) antibody. We required that <0.5% of the cells were positive with the isotype controls, and for all data reported in the article, we subtracted the signal of the isotype from the signal due to the specific antibodies. APC = allophycocyanin; IgG = immunoglobulin G; KDR = kinase insert domain receptor.
|
