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Figure 2 H₂O₂ Detected With DCF Fluorescence

Hydrogen peroxide (H₂O₂) in a normal (A) and stenotic (C) aortic valve detected with dichlorofluorescein (DCF) fluorescence. Levels of H₂O₂ were markedly elevated near the calcified regions of the valve, and most of the DCF fluorescence was eliminated by pre-incubation of the slide with polyethylene glycol (PEG)-catalase (CAT) (B and D). (E) The PEG-CAT–inhibitable fraction of DCF fluorescence in normal (base and tip regions) and stenotic (calcified and noncalcified regions) aortic valves (n = 4 normal valves, n = 7 stenotic valves; *p < 0.05 vs. noncalcified stenotic tissue, #p < 0.05 vs. base region of normal valves). Abbreviations as in Figure 1.

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