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Figure 1 EC–Specific Human Angiogenesis Assay
(A) Light photomicrograph (x20 original magnification) of endothelial cells (ECs) forming tubular structures (arrows) when co-cultured with MRC5 fibroblasts. (B) Immunofluorescent micrograph (x20 original magnification) of EC tubules, stained for 1,1'–dioctadecyl-3,3,3',3-tetramethyl-indocarbocyanine perchlorate labeled acetylated low-density lipoprotein (Dil-AcLDL) (red), Ulex europaeus agglutinin lectin (UEA-1) (green), and counterstained with 4 , 6-diamidino-2-phenylindole (DAPI) (blue). (C) Light photomicrograph (x10 original magnification) ECs stained for CD31 (brown) (arrows) failed to form tubules when cultured on smooth muscle cells.
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