Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 6 In Vitro MMP-9 Inhibition
(A) Representative Western blot data detecting the expression of matrix metalloproteinases (MMP)-9 and -2 proteins in macrophages (a). The control group (Co) markedly enhanced the expression of MMP-9 compared with native (Na). The 0.2- and 20-µmol/l clarithromycin (CAM) treatment (0.2 CA and 20 CA) altered the MMP-9 protein levels compared with the control group. However, MMP-2 expression was not altered by CAM treatment. (b) Representative gels showed the expression of MMP-9, MMP-2, and tissue inhibitor metalloproteinase (TIMP)-1 messenger ribonucleic acid (mRNA) levels by reverse transcriptase-polymerase chain reaction (RT-PCR). The CAM treatment altered the expression of MMP-9 but not MMP-2 and TIMP-1. The treatment of 20 µmol/l CAM inhibited expression of MMP-9 more effectively than that of 0.2 µmol/l. (B) Representative RT-PCR data detecting the expression of MMP-9, MMP-2, and TIMP-1 mRNA in the smooth muscle cells (SMCs). The control group markedly expressed MMP-9, and CAM treatment inhibited the expression of MMP-9 mRNA levels. The CAM treatment did not alter the MMP-2 and TIMP-1 expression. GAPDH = glyceraldehyde-3-phosphate dehydrogenase.
|
