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Figure 6 Expression Levels of Akt, ERK, and eNOS
(A) The rHDL activated p-Akt, p-ERK, and p-eNOS after 10 min of incubation in human coronary artery endothelial cells. (B) The rHDL-induced extracellular-signal-regulated kinase (ERK) activation was blocked by wartmannin and PD98059, but not L-NAME. The rHDL (5 µg/ml)-induced Akt activation was blocked by wartmannin (100 nmol/l), but not by PD98059 (3 µmol/l) or L-NAME (1 µmol/l). (C) The scavenger receptor class B, type I (SR-BI), adenosine triphosphate-binding cassette transporter (ABC) A1, or ABCG1 cells were transfected into ldlA7 cells. Forty-eight hours after transfection, the cells were cultured under serum-free conditions for an additional 24 h. The rHDL (5 µg/ml) was added to the medium for 10 min, and then the cells were lysed and analyzed. Representative pictures are shown (A to C). The graph shows the ratio of phospho- to total eNOS, Akt, and ERK in each sample relative to 1.0 for no treatment. *Significant increase (p < 0.05) versus no treatment. Abbreviations as in Figure 1.
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