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Figure 5 Western Blot of Phospo-p70S6 Kinase and Total p70S6 Kinase in an In Vivo Study
(Top) Western blot analysis of p70S6 kinase showing the effect of KATP channels on immunorecognition of p70S6 kinase in homogenates of the LV from the border zone. p70S6 kinase activity was measured with phospho-specific antibody against phosphorylated p70S6 kinase (top band) and a total p70S6 kinase antibody (bottom band). Ventricular remodeling after myocardial infarction was associated with marked increase of phospho-p70S6 kinase. A significantly reduced phospho-p70S6 kinase had taken place in the groups treated with either nicorandil or pinacidil administration compared with that seen in vehicle. (Bottom) Densitometric quantification of phosphorylation levels was expressed as the ratio of the density of phosphorylated band over total p70S6 kinase. Each point is an average of 3 separate experiments. *p < 0.007 compared with vehicle-, Glib-, Nic + Glib-, and Pin + Glib–treated groups. Abbreviations as in Figure 1.
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