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Figure 4 Hypoxia Detection in Arterial Wall and Human THP-1 Macrophages After Hypoxic and/or H2O2 Exposure
(A) Hypoxia (pimonidazole immunoreactivity) is almost absent in the arterial wall collected right after carotid incision, as well as right after excision of the atherosclerotic plaque (B). Insets show origin of magnification. (C) Pimonidazole was detected in human THP-1 macrophages in 0.2% O2 with fluorescence-activated cell sorting analysis (green bars), but was undetectable in 21% O2
(open bars) or after H2O2 stimulus (black bars). No significant differences were found between single O2 and O2 + H2O2 exposure (blue bars). *p < 0.05 versus 21% O2. Pimo-FITC = fluorescein isothiocyanate-conjugated antipimonidazole.
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