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Figure 2 SNP1192 and SNP1719 Influenced the Binding Efficiency of VEGF to KDR
HEK293s cells were transfected with 8 µg of pcDNA3.1-KDR (KDR-VH, KDR-IH, KDR-VQ, or KDR-IQ). After 36 h, the cells were rinsed with cold phosphate-buffered saline 3 times, and the binding of vascular endothelial growth factor (VEGF)165 (10 ng/ml, R&D Systems, Minneapolis, Minnesota) was carried out in binding buffer containing DMEM, 25 mmol/l HEPES (pH 7.4), 1 µg/ml heparin, and 0.1% gelatin for 2 h at 4°C. Then the cells were rinsed with cold phosphate-buffered saline 5 times, lysed with cell lysis buffer, followed by enzyme-linked immunosorbent assay. The values were the ratio of VEGF to KDR. The experiments were repeated 3 times, and 2 replicates were performed for each experiment. The values were presented as means, and the T bars represented standard deviations. Abbreviations as in Figure 1.
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