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Figure 6 Quantification of Infiltrated Cells in Plaques of PC-Immunized Mice
Immunohistochemistry was performed using anti–I-Ab (inflammatory cells) and anti-CD19 (B lymphocytes) primary antibodies. Positive cells were revealed by alkaline phosphatase-conjugated secondary antibodies and fast red substrate. Slides were counterstained by hematoxylin. Positive (red) staining was quantified by computer-assisted image analysis, and data are expressed as percentage of total cells. Inflammatory cells were significantly reduced in plaques of PC-immunized apolipoprotein E (apoE) knockout (KO) mice. In contrast, the number of infiltrating B lymphocytes was increased in PC-immunized apoE KO mice. *p < 0.05 versus PBS; **p < 0.01 versus KLH or PBS (1-way analysis of variance). Abbreviations as in Figure 1.
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