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Figure 4 Immune Sera Prevent oxLDL Uptake by Macrophages
Thioglycollate-elicited peritoneal macrophages were cultured on LabTek slide chambers during 24 h with complete DMEM medium supplemented with 10% fetal calf serum (FCS) and 20% L-929 conditioned medium. Macrophages were then deprived of FCS for 12 h before a 15-h incubation with or without oxidized low-density lipoprotein (50 µg/ml) and with or without serum (50 µg/ml) from phosphate-buffered saline (Ctrl) or PC-immunized mice (PC-KLH). Internalized LDL were stained with oil red O, and 3 random fields per condition were observed with phase-contrast microscopy (magnification x200). A customized program written with Qwin (Leica) was used to quantify the intracellular oil red O staining. The mean (±SEM) of intracellular staining per field is expressed in arbitrary units (au). A representative field and cell (frame) is shown for each condition. *p < 0.05 versus oxLDL without serum (1-way analysis of variance). Abbreviations as in Figures 1 and 3.
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