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Figure 1 Active Anti-PC Immunization Is Atheroprotective
Extent of lesion development was analyzed by computer-assisted morphometry on oil red O-stained and hematoxylin-counterstained serial sections of the aortic root. The immunization protocol started in 8-week-old female apolipoprotein E knockout mice and was scheduled every second week. Mice (n = 8/group) received intraperitoneal injections of either phosphate-buffered saline (PBS) or the adjuvant coupled to either keyhole limpet hemocyanin (KLH) or phosphorylcholine (PC)-KLH. Mice were killed after 4 months of treatment. (Top) Quantification of lesion size in cross-sections of the aortic root at the specified distance from the appearance of the first cusp. (Bottom) Representative micrographs of oil red O-stained images from the 3 groups at the specified distance from the appearance of the first cusp. *p < 0.01 versus PBS or KLH (2-way analysis of variance).
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