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Figure 5 Effects of rAAV/ASK ${Delta}$ N(KR) on Histological Abnormalities

Hematoxylin and eosin-stained (A to C) and Masson-trichrome-stained (D to F) sections of normal hamsters (A and D), rAAV/LacZ-treated (B and E), and rAAV/ASK ${Delta}$ N(KR)-treated hamsters (C and F). Each bar represents 100 µm. (G) mRNA levels of ${alpha}$ 1 type I and III collagen were evaluated by dot blot analysis (type I collagen: n = 3 for each group; type III collagen: n = 5 for normal hamsters, n = 4 for rAAV/LacZ or rAAV/ASK ${Delta}$ N(KR)). The ratio of ${alpha}$ 1 type I or III collagen to GAPDH in a normal hamster was expressed as 1. *p < 0.05 versus rAAV/LacZ, ${dagger}$ p < 0.05 versus normal hamsters. Abbreviations as in Figures 1 and 4.

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