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Figure 3 CD31Rg Binds to Plaque-Infiltrating Cells

Immunohistochemistry of CD31Rg in aortic root crysosections. CD31Rg (A), but not CD31Rg 1-2 (B), binds a consistent number of plaque-infiltrating cells. CD31Rg binding was detected on CD31^bright cells (white arrows) as well as on CD31^low cells (white arrow heads). CD31Rg staining was revealed by HRP/DAB (brown, A to B; green, C and F), nuclei were counterstained by hematoxylin (light blue, A to B) or DAPI (blue, E to F). CD31⁺ cells were visualized by a monoclonal rat antimouse CD31 antibody (MEC13.3) and an Alexafluor 555-conjugated secondary antibody (red, D and F). Original magnification 200x. A = adventitia; L = lumen; M = media; other abbreviations as in Figure 1.

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