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Figure 2 High Telomerase Activity in Coronary Plaque PMN of Patients With UA
Cell extracts derived from polymorphonuclear neutrophils (PMN) isolated from arterial (A) or venous blood (V) or directly from angioplasty balloons (AB) of unstable angina (UA) and chronic stable angina (SA) patients were assayed for telomerase activity by telomeric repeat amplification protocol assay in the presence of internal control (IC) (36 base pairs). As positive and negative controls, cell extracts (0.1 µg) from telomerase-positive HeLa cells were assayed before and after heat inactivation (HeLa and HeLa H.I.), respectively. Panels relative to the IC are shown at a lower detection exposure. Telomerase activity was barely detectable in PMN of patients with chronic SA (lanes 1 to 6, 13, and 14) and in arterial and venous PMN in patients with UA (lanes 8 and 9 and 11 and 12). In contrast, telomerase activity was high in PMN from the coronary atherosclerotic plaques in patients with UA (lanes 7 and 10).
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