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Figure 2 Re-Endothelialization, Macrophages, Transgene Expression, and Oxidant Production
(A) Endothelial coverage. (B) Neointimal macrophages. (C) LacZ transduced vessels and reverse transcriptase-polymerase chain reaction for extracellular superoxide dismutase (EC-SOD) mRNA. EC-SOD = 6-day EC-SOD–stented vessel; LacZ = 6-day control stented vessel. (D) DHE-stained nuclei in 6-day neointima and peroxynitrite (ONOO–)-modified epitopes. Representative images. Arrowheads = IEL. Bars = 100 µm. *p < 0.05. AdEC-SOD = adenovirus-encoding rabbit extracellular superoxide dismutase; Anti-β-gal-mAB = immunohistochemical statin with monoclonal antibody against β-galactosidase; DHE = dihydroethidium; HOCI = hypochlorite; LacZ = β-galactosidase; mAb 2D 10G9 = monoclonal antibody against hypochlorite modified epitopes; mw = marker; Neg. = reaction product without test sample; PECAM-1 = platelet endothelial cell adhesion molecule-1; Pos. = extracellular superoxide dismutase pDNA; X-gal = 5-brom-4-chlor-3-indoxyl-β-D-galactopyranoside chromogen substrate for β-galactosidase; other abbreviations as in Figure 1.
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