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Figure 4 Long-Term Survival and Maintenance of Cardiac Phenotype of the Transplanted hESC-CMs
(A) Polymerase chain reaction-based deoxyribonucleic acid amplification using human-specific primers at various time points after transplantation. (B) Coimmunostaining with anti-LacZ (red) and antitroponin I (green) antibodies (bar: 100 µm). (C) The nLacZ-expressing cells (blue) were isolated using laser microdissection (shown before [left] and after [right] laser microdissection) (bar: 200 µm). (D) Myosin light chain-2a expression in the microdissected nLacZ-expressing cells, in a remote left ventricular (LV) site, and in a saline-injected heart. Contracting embryoid bodies served as positive controls. d = days; hESC-CMs = human embryonic stem cell-derived cardiomyocytes.
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