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Figure 4 Effects of Cell Swelling on WT and R14C I _Ks Channels

(A) Hypotonic solution resulted in an increase in current amplitude (green) for both wild-type (WT) and R14C I _Ks channels. (B) After 5-min exposure to hypotonic solution, currents recorded at the end of a 1-s depolarization pulse to +20 mV increased to a greater extent in R14C (blue, 215 ± 34%) compared with WT (red, 96 ± 34%) I _Ks channels (WT vs. R14C, p < 0.05). (C) Hypotonic solution caused a greater increase in current density at all voltages for R14C compared with WT I _Ks channels. (D) Hypotonic solution caused a greater leftward shift in the V_0.5 of the voltage dependence of activation for R14C than for WT I _Ks channels (values for V_0.5 and k obtained from Boltzmann fits to the steady-state activation curves are summarized in ). Hypotonic solution caused an acceleration of the rates of activation (E) and deceleration of the rates of deactivation (F) for both WT and R14C I _Ks channels. However, the changes in values for _deact were more marked for R14C than for WT I _Ks channels. Symbols in panels D to F are the same as in Figure 3.

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