Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 3 Cyclosporin A, BAPTA-AM, TG, Ryanodine, or Nicotine Inhibit LPS-Induced Calcineurin Activity
Calcineurin activity in cardiac myocytes (mean + SE) increased after 16-h incubation with lipopolysaccharide (LPS) (10 ng/ml) compared with control (vehicle) (p < 0.05). (A) This was blocked by pre-treating myocytes (1 h before LPS) with either cyclosporin A (0.5 µmol/l), 1,2-Bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester (BAPTA-AM) (0.1 µmol/l), or thapsigargin (TG) (1 µmol/l) (n = 5). (B) Lipopolysaccharide effects were blocked by pre-treating myocytes with ryanodine (1 µmol/l, 1 h before LPS), or nicotine (15 ng/ml, 4 h before LPS) (n = 6).
|
