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Figure 2 Inhibition of CXCL16 Induction by Anti-Inflammatory Drugs
Down-regulation of (A) CXCL16 mRNA (F = 19.4, p = 0.001) and (B) CXCL16 protein secretion (F = 71.9, p < 0.001) by aspirin (ASA) in human macrophages. Cells were pre-treated with aspirin for 2 h and then for 24 h with LPS (1 µg/ml). **p < 0.01, ***p < 0.001 by t test compared to LPS-induced CXCL16 levels. (C) Down-regulation of CXCL16 mRNA in human macrophages by the nuclear factor-kappa-B inhibitor SN50 (t test p = 0.002). Cells were pretreated with SN50 or control peptide at 100 µg/ml for 2 h, then for 24 h with LPS (1 µg/ml). (D) Induction of CXCL16 in human macrophages by adenovirus over-expression (24 h) of activated I-kappa-B kinase compared to control virus (*t test p = 0.03). Results of representative experiments with triplicate samples are expressed as mean ± (SEM). GFP = green fluorescent protein; other abbreviations as in Figure 1.
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