Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 4 PCR on Complementary Deoxyribonucleic Acid
Reverse transcription polymerase chain reaction (RT-PCR) on ribonucleic acid (RNA) extracted from fibroblasts of patient III-12 and from lymphocytes of a control individual. The RNA of the patient show, in addition to the normal-sized band, a shorter product that after sequencing proved to have lost both exons –1 and +1. The normal-sized product is weaker, owing to the fact that shorter amplicons are more abundantly amplified.
|
