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Figure 3 Characterization of 
-SMA–Positive Cells
Smooth muscle myosin heavy chain isotype II (SMMHC-II) (A) immunohistochemical staining colocalized with alpha-smooth muscle actin (-SMA)–positive cells suggesting mature contractile ability. Electron micrographs (B to D) of the muscle-like bundles beneath the polyester urethane urea patch exhibited ultrastructural features typical of mature contractile phenotype smooth muscle cells. White arrow denotes caveole. Black arrow indicates dense bodies. Scale bars for A are 20 µm, for B are 2 µm, and for C and D are 100 nm. My = myofibril; N = nuclear.
