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Figure 4 Ca2+ Leak and Interdomain Interaction Within RyR2
(A, top) Representative time courses of Ca2+ uptake and Ca2+ leak from SR vesicles in presence of SIN-1 (100 µmol/l), CV (100 µmol/l), or DPc10 (30 µmol/l). Summarized data of Ca2+ leak (bottom). (B) Stern-Volmer plots of the fluorescence quenching data with QSY-bovine serum albumin (BSA). The top panel shows the effect of SIN-1 (100 µmol/l) in the presence or absence of CV (100 µmol/l) or CV (100 µmol/l) plus DPc10 (30 µmol/l) in normal SR. Bottom panel shows Stern-Volmer plots of QSY-BSA fluorescence quenching data of normal, failing (CV-untreated/paced), and CV-treated/paced groups. (C) Effect of SIN-1 (100 µmol/l) on phosphorylation level of RyR2-pSer2808
(top) or amount of RyR2-bound FKBP12.6 (bottom), in the presence of CV (100 µmol/l) or DPc10 (30 µmol/l). Before immunoprecipitation of RyR, SR vesicles were mixed with CV or DPc10 for 30 min and then centrifuged, followed by Western blotting. Abbreviations as in Figures 2 and 3.
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