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Figure 2 Redox State in RyR2
(A) Monobromobimane (mBB) fluorescence intensity of the ryanodine receptor (RyR)2 (corresponding to the content of free thiols) in the presence or absence of 100 µmol/l 3-morpholinosydnonimine (SIN-1). The relative content of free thiols in the RyR2 was obtained as the ratio of fluorescence intensity of the mBB to protein abundance of RyR2 and then expressed as a percentage of that in normal sarcoplasmic reticulum. (B) Acute concentration effect of carvedilol (CV) on SIN-1 (100 µmol/l)–induced decrease in mBB fluorescence intensity of the RyR2. (C) Acute effect of CV (100 µmol/l) on the mBB fluorescence intensity of the RyR2 in the presence or absence of SIN-1 (100 µmol/l) and/or DPc10 (a synthetic peptide corresponding to Gly2460-Pro2495 of RyR) (30 µmol/l).
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