Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 2 eNOS Expression After Retroinfusion of eNOS S1177D
Compared with a control heart (A), endothelial nitric oxide synthase (eNOS) S1177D transfection enhanced eNOS expression in the ischemic area (B). (C) In comparison, inducible NOS (iNOS) was found to be more confined to round cells (arrows), which did not express eNOS (D = merged fluorescence). Red bar = 50 µm. (E) Immunoblotting of eNOS revealed a 2.3-fold increase of eNOS expression in the ischemic region, as compared with the control region (representative example of 3 experiments). (F) Cyclic guanosine monophosphate (cGMP) levels detected by radioimmunoassay (RIA) indicate an increased cGMP formation in eNOS S1177D-treated animals, except for L-nitroarginine-methylester (L-NAME) co-application (normalized to nonischemic tissue, 3 animals/group with 3 tissue specimens/condition each). LAD = left anterior descending artery; RCx = ramus circumflexus.
|
