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Figure 3 Effect of naloxone treatment on LPS-induced macrophage production of superoxide. The THP-1 cell culture was pretreated for 1 h with the indicated concentrations of naloxone before stimulation with 100 ng/ml LPS. Production of superoxide in THP-1 cell culture was measured by lucigenin-enhanced chemiluminescence. Data are expressed as the mean values ± SD of 3 experiments. *p < 0.05 compared with the LPS-treated cultures. RLU = relative light units; other abbreviations as in Figure 1
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