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Figure 3 SPM-labeled EOCs: interactions with magnetic fields. (A and B)
Stacked confocal images obtained along the z-axis (100x magnification). EOCs are labeled with CM-DiI (red)
and SPM (green)
. Exposure of SPM-loaded EOCs to a degaussed stainless steel loop does not result in significant cell attraction (A)
. On the contrary, in the presence of a magnetized stainless steel loop, SPM-loaded cells are rapidly cleared from the suspension and accumulate predominantly on the loop bend (B)
. A green reflection from the stainless-steel loop is evident. (C and D)
Nickel coating of a commercially available stent results in multiple, small, uniformly distributed magnetic domains, as shown in ferrofluid studies (C)
. Presence of numerous magnetic microdomains is evident (arrows)
. This translated into a more uniform coverage of the stent surface (D)
. Endothelial outgrowth cells are labeled with SPM (green)
. (E and F)
Transmission electronic microscopy confirms uniform cytoplasmic capture of SPM microspheres (E)
. Scanning electron microscopy shows early accumulation of rounded cells to the metal surface (F)
. (G and H)
Representative en face fluorescent microscopy images (200x magnification) from explanted coronary arteries. Local delivery of CM-DiI (red)
–labeled EOCs to a non-magnetized stent containing arterial segment results in retention of cells in small numbers (G)
. Local delivery is greatly enhanced in the segment that received a nickel-coated, magnetized stent before EOC delivery. Note presence of CM-diI–labeled cells along a stent strut (H)
. Abbreviations as in Figure 1
.
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