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Figure 2 Apoptosis and secretory profiles are stable with low-dose SPM. (A and B)
Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assays: composite confocal images. Green fluorescence is given by SPM whereas red fluorescence represents TUNEL-positive apoptotic cells. Exposure to SPM at a 500:1 ratio results in minimal apoptosis (A)
, whereas significantly increased presence of apoptotic cells is evident at a higher SPM to EOC ratio (B
, 4,000:1 ratio). (C and D)
Cytokine array studies. EOC secretory profile was very similar for untreated cells (C)
and cells exposed to SPM at a 500:1 ratio (D)
. The top four left dots
and bottom two right dots
are built-in positive controls of the array. All other dots
represent an individual cytokine (72 in total). With these studies, secretion of interleukins (IL)-8 and IL-10; growth factors EGF, HGF, and GDNF; and metalloproteinase inhibitors TIMP-1 and TIMP-2 and MIP-1b were identified. Other abbreviations as in Figure 1
.
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