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Figure 3 Cluster analysis of mouse myocardial peptide fractions emerging from "shotgun" processing. The peptides naturally cluster into cytosolic, microsomal, mitochondrial, and nuclear fractions. The peptide localization confers functional specificity, such that the calcium regulatory proteins are much more abundant in the microsomal fraction, whereas transcription factors are in the nuclear-specific fractions. Ca2+
= calcium; GO = Gene Ontology; MudPIT = multidimensional protein identification technology.
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