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Figure 2 "Shotgun" proteomic analysis combining liquid chromatography with mass spectrometry (LC-MS) or so-called multidimensional protein identification technology (MudPIT). In this approach, proteins are enzymatically digested and subjected to fractionation through cation exchange and reverse phase, and the peptide fragments are sprayed through high voltage into the mass spectrometer. The unique emerging spectra are then recorded and compared with existing databases to search for their identity. HPLC = high-pressure liquid chromatography; MS = mass spectometry; RP = reverse phase; SCX = strong cation exchange.
