Please click here to obtain permission to reproduce this image.
Click on image to view larger version.
Figure 1 Large-scale protein analysis of myocytes can be done with 2-dimensional gel electrophoresis (2-DE), where the proteins can be separated and identified on the basis of molecular weight and pH. However, to improve the resolution and to achieve greater protein separation, optimization of the gel processing conditions (e.g., expanding the pH range by selective isoelectric [IE] focusing) helps to improve the resolution significantly (for details, see Stanley et al. [37
]). ASB = amidosulfobetaine; SDS PAGE = sodium dodecylsulfate polyacrylamide gel electrophoresis.
