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Figure 5 Changes of glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidase (Gpx), and glutathione reductase (GR) contents or activity in the diabetic hearts and MT’s effect. The GSH and GSSG contents were measured by high-performance liquid chromatography with a dual electrochemical method from the cytosolic (A) and mitochondrial (B) fractions of the control and diabetic tissues from WT and MT-TG diabetic mice. The Gpx and GR activities (C) were measured by biochemical assay using commercially available kits and visible spectrometer. Data were presented as mean values ± SD. Animal numbers are 4 to 5 in each group. A 2-tailed unpaired Student t test was used except for the right panel of B, for which, because the variables seriously violated the assumptions for normality and homogeneity of variances, a Welch t test was used. *p < 0.05 versus corresponding control; #p < 0.05 versus WT control. Other abbreviations as in Figure 1.
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