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Figure 5 The effects of different concentrations of recombinant human neutrophil-activating peptide (rhNAP)-2 on E-selectin (A), vascular cellular adhesion molecule (VCAM)-1 (B), monocyte chemoattractant protein (MCP)-1 (C), and interleukin (IL)-8 (D) in human umbilical vein endothelial cells after culturing for 5 (A and B) and 24 h (C and D). (E) Shows the effect of neutralizing antibodies against CXCR1 and CXCR2 (p = 0.06) receptors on the rhNAP-2-induced (250 ng/ml) release of IL-8 in human umbilical vein endothelial cells supernatants after culturing for 24 h. No down-regulatory effect was seen when a control antibody of the same isotype and concentration was added (data not shown). (F) Shows the ability of a neutralizing antibody against NAP-2 and a control antibody of the same isotype and concentration (IgG1), to attenuate the effect of the platelet extract-mediated increase of IL-8 release in human umbilical vein endothelial cell supernatants after culturing for 5 h. Data are mean values ± SEM of 6 separate experiments. *p < 0.05 and **p < 0.001 versus unstimulated (Unstim) cells; #p < 0.05 versus cells stimulated with rhNAP-2 (E) or platelet extract (PE) (F). The Wilcoxon signed rank test (simple comparisonwise p values) was used for statistical comparisons. OD = optical density.
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