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Figure 8 Cholesterol efflux from cholesterol-loaded macrophages. Transduced RAW cells expressing genes for green fluorescent protein (GFP), apolipoprotein A-I (A-I), or apolipoprotein A-I Milano (A-IM) were used for cholesterol efflux in the absence of exogenous extracellular acceptors. In addition, nontransduced RAW cells were loaded with 3H-cholesterol, and then cholesterol efflux was measured 4 h after loading in the presence of dipalmitoyl phosphatidylcholine (DPPC) alone or DPPC with 200 µg/ml of either apoA-I (A-I) or apoA-IMilano (A-IM). A similar experiment was performed with the peritoneal macrophages that were isolated from apoA-I–/–/apoE–/– double-knockout mice. Each bar shows the mean ± SD of a triplicate determination. The experiments were repeated twice with similar results.
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