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Figure 3 Immunocytochemical analysis of circulating cells. (A) Buffy-coat smear-stained with P1H12 showing a circulating endothelial cell (red staining) (magnification x1,000) (left). A circulating endothelial cell stained for myeloid-related protein (MRP)-8/MRP-14 (green staining) (magnification x1,000) (center) in acute Kawasaki disease (KD) patients with coronary artery lesions (CAL). Nuclei stained with DAPI (magnification x1,000 (right). (B) Quantitation of circulating endothelial cells (left) and cells positive for MRP-8/MRP-14 (right) in patients with acute KD with or without CAL. *p < 0.05 patients without CAL versus patients with CAL. Black columns = patients with CAL (n = 9); white columns = patients without CAL (n = 15).
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